Receptor Localization: Laboratory Methods and Procedures

Examination of the immunohistochemical localization and gene expression by RT-PCR of the oxytocin receptor in diabetic and non-diabetic mouse testis

Objective(s): The aim of this study was to determine Oxytocin receptor (OTR) gene expression and localization in diabetic and non-diabetic mouse testes by RT-PCR and immunohistochemistry, respectively. Materials and Methods: In this study, 18 male BALB/c mice (8–12 weeks old) were used and divided into three groups: diabetic, sham, and control. Streptozotocin (STZ) was applied to the diabetic g...

Concerning the need for more sophisticated animal models in sensory behavioral toxicology.

It is necessary but not sufficient to develop laboratory animal models in sensory behavioral toxicology for screening toxic substances and for the analysis of sensory impairment at threshold levels of stimulation. It is important to develop more thorough and quantitative tests of impairment which in their greater complexity more accurately reflect the conditions and environmental demands of day...

Assessment of Ultrasound / Radio-guided Occult Lesion Localization in Non-palpable Breast Lesions

Objective(s): Controversy exists about the localization of non-palpable breast lesions. In many countries, the gold standard for the diagnosis of these lesions is needle localization due to its accuracy. This study sought to compare the ultrasoundand radio-guided occult lesion localization (ROLL) as a simple method with the conventional procedures in terms of their diagnostic power. Methods: Th...

A New Paradigm in Biology – The Effect of Localization of a Protein on its Anti-genicity

Immunohistochemical Localization of Ghrelin in Testicular Tissues of Holstein Bulls

Ghrelin, a 28-amino acid acylated peptide, was identified as the endogenous ligand for the growth hormone (GH) secretagogue receptor and its role in reproduction has recently been established. It is believed that the site of ghrelin expression in testes may indicate its role in local regulation. This study investigated the immunohistochemical (IHC) localization of ghrelin in Holstein bull testi...